AB172. Low-intensity pulsed ultrasound recovers erectile function in streptozotocin-induced type I diabetic rats

© Translational Andrology and Urology. All rights reserved. Transl Androl Urol, 2015;4(S1) www.amepc.org/tau corpus cavernosa in the horizontal direction. In the CD rat, we observed the tissue of the regenerated corpus cavernosa, the structure resembled that of the SH rat in appearance of HE-stained tissue. Immunohistochemical analysis revealed that, in the SH rat, vascular smooth muscles were detected in the central part of the corpus cavernosum penis, and neuron tissues were detected in the lateral sides of venous sinus. In the EX rat, the regenerated vascular smooth muscles and neuron tissues were hardly detected. In the AL rat, vascular smooth muscles and neuron tissues were regenerated in the horizontal direction, but their constructions were different from those of the SH rat. Similarly, in the CD rat, some vascular smooth muscles were detected. Moreover some venous sinus-like structures without vascular smooth muscles were detected and neuron tissues were regenerated in the lateral sides of the structures. This venous sinus-like stricture was assumed to be an immature venous sinus. The RT-PCR for the extracts from the alginate gel sponge sheets retrieved in the CD group revealed that qRT-PCR helped in determining the amounts of mRNA for rat NGF and rat VEGF to be significantly higher than those in the AL group (NGF: P=0.0309, VEGF: P<0.0001). These results indicate that upregulation of intrinsic growth factors can be derived from rats. Conclusions: Transplantation of CD133 cells accelerated the functional and histological recovery in this corpus cavernosa defect model . We bel ieve CD133 cel l transplantation will be able to be applied to the treatment of penile regeneration or severe ED that is not responsive to PDE5 inhibitors. The CD133 cells can be isolated easily from peripheral blood and applied as an autologous therapeutic agent with no limitation due to ethical or technical problems. This advantage will accelerate the progression of cell therapies for penile regeneration from the laboratory to clinical implementation.